pJK317
(Plasmid
#72472)
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Purpose(Empty Backbone) puc118 modified to contain ribosome binding site
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC118
- Backbone size (bp) 3175
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Modifications to backboneAdds a ribosome binding site and NdeI site to insert genes. This plasmid is missing nucleotide C222 from the canonical pUC118 sequence, which disrupts the M13-rev primer site.
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Vector typeBacterial Expression
- Promoter lac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-forward
- 3′ sequencing primer none (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The M13R sequence is non-standard and cannot be used for sequencing with the normal primer.
* 5′ cloning siteEcoRI (not destroyed during cloning)
* 3′ cloning siteBamHI (destroyed during cloning)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK317 was a gift from T. J. Kappock (Addgene plasmid # 72472 ; http://n2t.net/addgene:72472 ; RRID:Addgene_72472)
