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Addgene

pJK646
(Plasmid #72469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3529
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    response regulator in two-component regulatory system with EvgS
  • Alt name
    evgA
  • Species
    Escherichia coli str. K-12 substr. MG1655
  • GenBank ID
    NP_416870.1
  • Entrez Gene
    evgA (a.k.a. b2369, ECK2365)
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13-reverse
  • 3′ sequencing primer M13-forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert is oriented antiparallel to lac promoter. 5'-UTR contains EvgA binding site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK646 was a gift from T. J. Kappock (Addgene plasmid # 72469 ; http://n2t.net/addgene:72469 ; RRID:Addgene_72469)
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