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Addgene

pJK640
(Plasmid #72467)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDRIVE-1
  • Backbone size w/o insert (bp) 3851
  • Total vector size (bp) 4765
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    response regulator in two-component regulatory system with EvgS
  • Alt name
    evgA
  • Species
    Escherichia coli str. K-12 substr. MG1655
  • GenBank ID
    NP_416870.1
  • Entrez Gene
    evgA (a.k.a. b2369, ECK2365)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TA cloning product with 286 bp 5'-UTR including EvgA binding site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK640 was a gift from T. J. Kappock (Addgene plasmid # 72467 ; http://n2t.net/addgene:72467 ; RRID:Addgene_72467)