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Addgene

pJK552
(Plasmid #72451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDFDuet-1
  • Backbone size w/o insert (bp) 3781
  • Total vector size (bp) 5436
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide mutase
  • Alt name
    purE
  • Alt name
    AZ09_02695
  • Species
    Acetobacter aceti 1023
  • Mutation
    inserted Val2
  • GenBank ID
    AAZ04483.1 KDE21030.1
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide synthetase
  • Alt name
    purK
  • Alt name
    AZ09_02690
  • Species
    Acetobacter aceti 1023
  • Mutation
    Val319 to Phe
  • GenBank ID
    AAZ04484.1 KDE21030.1
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer DuetUP2
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK552 was a gift from T. J. Kappock (Addgene plasmid # 72451 ; http://n2t.net/addgene:72451 ; RRID:Addgene_72451)
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