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Addgene

pJK521
(Plasmid #72440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72440 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 2631
  • Modifications to backbone
    Excised NdeI(vector)-NdeI(insert) segment to delete half of MCS.
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hypothetical protein
  • Alt name
    orfX
  • Alt name
    AAZ04482
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene with ~40 silent substitutions
  • GenBank ID
    AAZ04482.1 KDE21028.1
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer none (AmpR)
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK521 was a gift from T. J. Kappock (Addgene plasmid # 72440 ; http://n2t.net/addgene:72440 ; RRID:Addgene_72440)
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