pJK637
(Plasmid #72437)

• Purpose: Acetobacter aceti 1023 mutS, partial gene
• Depositing Lab: T. J. Kappock
• Publication: Acetobacter aceti 1023 genetic tools (unpublished)

Backbone

• Vector backbone: pDRIVE-1
• Backbone size w/o insert (bp): 3851
• Total vector size (bp): 5215
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DNA mismatch repair protein MutS (partial gene)
• Alt name: mutS
• Alt name: AZ09_07465
• Species: Acetobacter aceti 1023
• Mutation: deletes nt 1-767 and nt 2086-2655 of mutS gene
• GenBank ID: KDE20146
• Promoter: n/a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used for nucleotide quantitation in RT-PCR and similar experiments for Acetobacter aceti 1023.

Based on method described in M. Ishikawa, A. Okamoto-Kainuma, T. Jochi, I. Suzuki, K. Matsui, T Kaga, Y. Koizumi (2009) Cloning and characterization of grpE in Acetobacter pasteurianus NBRC 3283. J. Biosci. Bioeng. 109(1), 25–31[doi: 10.1016/j.jbiosc.2009.07.008]