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pJK215
(Plasmid #72427)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72427 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lambda-BlueStar
  • Backbone size w/o insert (bp) 2111
  • Total vector size (bp) 11599
  • Vector type
    phage lambda cloning vector with genomic DNA insert

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    succinyl-diaminopimelate desuccinylase
  • Alt name
    dapE
  • Alt name
    AZ09_12540
  • Species
    Acetobacter aceti 1023
  • Mutation
    missing C-terminal domain residues 277-391(ter)
  • GenBank ID
    KDE19320
  • Promoter n/a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sau3AI (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer T3 promoter
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
  • Alt name
    dapE
  • Alt name
    AZ09_12545
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19321
  • Promoter n/a

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    acetylglutamate kinase
  • Alt name
    argB
  • Alt name
    AZ09_12550
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19322
  • Promoter n/a

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    ribosome biogenesis GTP-binding protein YsxC
  • Alt name
    engB
  • Alt name
    AZ09_12555
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19323
  • Promoter n/a

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    insertase
  • Alt name
    yidC
  • Alt name
    AZ09_12560
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19324
  • Promoter n/a

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    ribonuclease P
  • Alt name
    AZ09_12565
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19325
  • Promoter n/a

Cloning Information for Gene/Insert 6

Gene/Insert 7

  • Gene/Insert name
    50S ribosomal protein L34
  • Alt name
    rpmH
  • Alt name
    AZ09_12570
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19326
  • Promoter n/a

Cloning Information for Gene/Insert 7

Gene/Insert 8

  • Gene/Insert name
    hypothetical protein
  • Alt name
    AZ09_12575
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19327
  • Promoter n/a

Cloning Information for Gene/Insert 8

Gene/Insert 9

  • Gene/Insert name
    glyoxylase I
  • Alt name
    gloA
  • Alt name
    AZ09_12580
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19328
  • Promoter n/a

Cloning Information for Gene/Insert 9

Gene/Insert 10

  • Gene/Insert name
    hypothetical protein
  • Alt name
    AZ09_12585
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19329
  • Promoter n/a

Cloning Information for Gene/Insert 10

Gene/Insert 11

  • Gene/Insert name
    UDP-N-acetylglucosamine acyltransferase
  • Alt name
    lpxA
  • Alt name
    AZ09_12590
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19330
  • Promoter n/a

Cloning Information for Gene/Insert 11

Gene/Insert 12

  • Gene/Insert name
    3-hydroxyacyl-ACP dehydratase
  • Alt name
    fabZ
  • Alt name
    AZ09_12595
  • Alt name
    (3R)-hydroxymyristoyl-dehydratase
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE19331
  • Promoter n/a

Cloning Information for Gene/Insert 12

Gene/Insert 13

  • Gene/Insert name
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
  • Alt name
    lpxD
  • Alt name
    AZ09_12600
  • Species
    Acetobacter aceti 1023
  • Mutation
    missing residues 1-280
  • GenBank ID
    KDE19332
  • Promoter n/a

Cloning Information for Gene/Insert 13

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site Sau3AI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Restores a D-Ala auxotroph to prototrophy on minimal medium. Recovered independently more than once.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK215 was a gift from T. J. Kappock (Addgene plasmid # 72427 ; http://n2t.net/addgene:72427 ; RRID:Addgene_72427)
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