pJK215
(Plasmid #72427)

• Purpose: Acetobacter aceti 1023 gDNA
• Depositing Lab: T. J. Kappock
• Publication: Additional Acetobacter aceti 1023 ORFs associated with functional complementation of an alanine racemase (alr-, dadX-) auxotroph (unpublished)

Backbone

• Vector backbone: lambda-BlueStar
• Backbone size w/o insert (bp): 2111
• Total vector size (bp): 11599
• Vector type: phage lambda cloning vector with genomic DNA insert

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: succinyl-diaminopimelate desuccinylase
• Alt name: dapE
• Alt name: AZ09_12540
• Species: Acetobacter aceti 1023
• Mutation: missing C-terminal domain residues 277-391(ter)
• GenBank ID: KDE19320
• Promoter: n/a

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sau3AI (not destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: T3 promoter
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: 2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
• Alt name: dapE
• Alt name: AZ09_12545
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19321
• Promoter: n/a

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: acetylglutamate kinase
• Alt name: argB
• Alt name: AZ09_12550
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19322
• Promoter: n/a

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: ribosome biogenesis GTP-binding protein YsxC
• Alt name: engB
• Alt name: AZ09_12555
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19323
• Promoter: n/a

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: insertase
• Alt name: yidC
• Alt name: AZ09_12560
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19324
• Promoter: n/a

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 6

• Gene/Insert name: ribonuclease P
• Alt name: AZ09_12565
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19325
• Promoter: n/a

Cloning Information for Gene/Insert 6

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 7

• Gene/Insert name: 50S ribosomal protein L34
• Alt name: rpmH
• Alt name: AZ09_12570
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19326
• Promoter: n/a

Cloning Information for Gene/Insert 7

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 8

• Gene/Insert name: hypothetical protein
• Alt name: AZ09_12575
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19327
• Promoter: n/a

Cloning Information for Gene/Insert 8

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 9

• Gene/Insert name: glyoxylase I
• Alt name: gloA
• Alt name: AZ09_12580
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19328
• Promoter: n/a

Cloning Information for Gene/Insert 9

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 10

• Gene/Insert name: hypothetical protein
• Alt name: AZ09_12585
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19329
• Promoter: n/a

Cloning Information for Gene/Insert 10

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 11

• Gene/Insert name: UDP-N-acetylglucosamine acyltransferase
• Alt name: lpxA
• Alt name: AZ09_12590
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19330
• Promoter: n/a

Cloning Information for Gene/Insert 11

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 12

• Gene/Insert name: 3-hydroxyacyl-ACP dehydratase
• Alt name: fabZ
• Alt name: AZ09_12595
• Alt name: (3R)-hydroxymyristoyl-dehydratase
• Species: Acetobacter aceti 1023
• GenBank ID: KDE19331
• Promoter: n/a

Cloning Information for Gene/Insert 12

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 13

• Gene/Insert name: UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
• Alt name: lpxD
• Alt name: AZ09_12600
• Species: Acetobacter aceti 1023
• Mutation: missing residues 1-280
• GenBank ID: KDE19332
• Promoter: n/a

Cloning Information for Gene/Insert 13

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: Sau3AI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Restores a D-Ala auxotroph to prototrophy on minimal medium. Recovered independently more than once.