pJK408
(Plasmid #72422)

• Purpose: Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag
• Depositing Lab: T. J. Kappock
• Publication: Additional Acetobacter aceti 1023 hydrolase/transferase enzymes (unpublished)

Backbone

• Vector backbone: pET23d
• Backbone size w/o insert (bp): 3663
• Total vector size (bp): 4634
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: feruloyl esterase
• Alt name: est10
• Alt name: AAC1141
• Alt name: AZ09_06910
• Species: Acetobacter aceti 1023
• GenBank ID: KDE20551.1
• Promoter: T7
• Tag / Fusion Protein:
  • His5 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct was prepared by QuikChange mutagenesis, which led to the unintended deletion of one codon in the terminal affinity tag, which now encodes His5.