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Addgene

pJK407
(Plasmid #72421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4634
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    lipase
  • Alt name
    est06
  • Alt name
    AZ09_08580
  • Alt name
    AAC0842
  • Species
    Acetobacter aceti 1023
  • Mutation
    lacks Met1 and Thr2 in annotated (v1) sequence
  • GenBank ID
    KDE19924.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK407 was a gift from T. J. Kappock (Addgene plasmid # 72421 ; http://n2t.net/addgene:72421 ; RRID:Addgene_72421)
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