pJK650
(Plasmid #72400)

• Purpose: Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
• Depositing Lab: T. J. Kappock
• Publication: Purine biosynthesis (unpublished)

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: glutamine synthetase
• Alt name: glnA
• Alt name: JW3841
• Species: Escherichia coli str. K-12 substr. MG1655)
• Mutation: Mutated tyrosine-397 to phenylalanine to block adenylylation (note:initiator Met is codon zero)
• Entrez Gene: glnA (a.k.a. b3870, ECK3863, JW3841)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene amplified from a plasmid template provided by Jamie Williamson (Scripps Research Institute) and cloned by our lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry