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Addgene

pJK621
(Plasmid #72388)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6583
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phosphoribosylamine-glycine ligase
  • Alt name
    purD
  • Alt name
    ECD_03882
  • Alt name
    glycinamide ribonucleotide synthetase
  • Species
    Escherichia coli BL21(DE3)
  • Mutation
    contains 3 silent mutations in codons 9-11 to facilitate PCR amplification
  • GenBank ID
    ACT45674.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK621 was a gift from T. J. Kappock (Addgene plasmid # 72388 ; http://n2t.net/addgene:72388 ; RRID:Addgene_72388)
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