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Addgene

pJK034
(Plasmid #72383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23d
  • Backbone size w/o insert (bp) 3663
  • Total vector size (bp) 4858
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    phosphoribosylglycinamide synthetase
  • Alt name
    purD
  • Alt name
    phosphoribosylamine-glycine ligase
  • Alt name
    glycinamide ribonucleotide synthetase
  • Species
    Bacillus subtilis
  • GenBank ID
    CAB12473.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    JoAnne Stubbe (MIT)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK034 was a gift from T. J. Kappock & JoAnne Stubbe (Addgene plasmid # 72383 ; http://n2t.net/addgene:72383 ; RRID:Addgene_72383)
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