pJK694
(Plasmid
#72379)
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PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic gene
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72379 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep425_GPD
- Backbone size w/o insert (bp) 7760
- Total vector size (bp) 9314
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namephosphoribosyl pyrophosphate amidotransferase
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SpeciesH. sapiens (human)
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Insert Size (bp)1554
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GenBank IDAAC27345.1
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Entrez GenePPAT (a.k.a. ATASE, GPAT, PRAT)
- Promoter GPD
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK694 was a gift from T. J. Kappock (Addgene plasmid # 72379 ; http://n2t.net/addgene:72379 ; RRID:Addgene_72379)