pFETCh_SSRP1
(Plasmid #72370)

• Purpose: Donor vector for 3' FLAG tag of human SSRP1
• Depositing Labs: Eric Mendenhall, Richard M. Myers
• Publication: Mendenhall CRISPR tagging plasmids (unpublished)

Backbone

• Vector backbone: pFETCh_Donor
• Backbone size w/o insert (bp): 5771
• Vector type: CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SSRP1 Homology Arms
• Species: H. sapiens (human)
• Entrez Gene: SSRP1 (a.k.a. FACT, FACT80, T160)
• Tag / Fusion Protein:
  • 3XFLAG-P2A-NeoR (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Neo-F
• 3′ sequencing primer: Neo-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositing laboratory notes that this clone grows slowly and the liquid culture may need to be incubated a little longer than usual. Importantly, they note that even though the clone grows slowly, the plasmid works in CRISPR experiments. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.