pFETCh_DNMT3B
(Plasmid
#72365)
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PurposeDonor vector for 3' FLAG tag of human DNMT3B
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72365 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFETCh_Donor
- Backbone size w/o insert (bp) 5771
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Vector typeCRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDNMT3B Homology Arms
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SpeciesH. sapiens (human)
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Entrez GeneDNMT3B (a.k.a. FSHD4, ICF, ICF1, M.HsaIIIB)
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Tag
/ Fusion Protein
- 3XFLAG-P2A-NeoR (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Neo-F
- 3′ sequencing primer Neo-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFETCh_DNMT3B was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 72365 ; http://n2t.net/addgene:72365 ; RRID:Addgene_72365)
