CY026
(Bacterial strain #72340)

• Purpose: Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR
• Depositing Lab: Matthew Bennett
• Publication: Chen et al Science. 2015 Aug 28;349(6251):986-9. doi: 10.1126/science.aaa3794.

Backbone

• Vector backbone: E. coli BW25113
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): CY026
• Growth instructions: One CmR resistance in genome.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: RhlR
• Insert Size (bp): 966
• Entrez Gene: rhlR (a.k.a. PA3477)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: CinR
• Insert Size (bp): 726
• GenBank ID: AF210630.1

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an ancestor strain of CY027. The chloramphenicol resistance marker can be removed by Flp-FRT recombination to obtain CY027.