pDsRed-TSTEP_v2
(Plasmid
#72336)
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PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II KS
- Backbone size w/o insert (bp) 2942
- Total vector size (bp) 6591
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
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Alt nameT-STEP cassette
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Insert Size (bp)1436
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDsRed-TSTEP_v2 was a gift from Avital Rodal (Addgene plasmid # 72336 ; http://n2t.net/addgene:72336 ; RRID:Addgene_72336) -
For your References section:
Tissue-specific tagging of endogenous loci in Drosophila melanogaster. Koles K, Yeh AR, Rodal AA. Biol Open. 2015 Dec 23. pii: bio.016089. doi: 10.1242/bio.016089. 10.1242/bio.016089 PubMed 26700726
Map uploaded by the depositor.
