pTSTEP
(Plasmid #72334)

• Purpose: Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)
• Depositing Lab: Avital Rodal
• Publication: Koles et al Biol Open. 2015 Dec 23. pii: bio.016089. doi: 10.1242/bio.016089.

Backbone

• Vector backbone: pTL2
• Backbone manufacturer: PMID: 25555988
• Backbone size w/o insert (bp): 7580
• Total vector size (bp): 9740
• Modifications to backbone: gRNA cloning site changed to BsmBI from SapI. I-CreI and attPx sites removed.
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RRS-TagRFPT-p10-[Golic+] RRS-EGFP
• Alt name: T-STEP cassette
• Insert Size (bp): 1436

Resource Information

• Supplemental Documents:
  • pTSTEP
• A portion of this plasmid was derived from a plasmid made by: pTL2 backbone from Chen et al, 2015, PMID: 25555988

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry