pBTR(SMCytc)
(Plasmid
#72330)
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PurposeExpresses spider monkey cytochrome c along with the heme lyase (CYC3) from yeast for heme insertion
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBTR1
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Backbone manufacturerCustom academic
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 5600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namespider monkey cytochrome c gene
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SpeciesAteles sp.
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Insert Size (bp)321
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CCGGGGATCCATAACTAAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGary Pielak University of North Carolina. pBTR(SMCytc) is derived from pBTR(HumanCc) by mutagensis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Olteanu et al. Biochem. Biophys. Res. Commun. 312, 733-740 (2003)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTR(SMCytc) was a gift from Bruce Bowler (Addgene plasmid # 72330 ; http://n2t.net/addgene:72330 ; RRID:Addgene_72330)