pET21 MBPx-SpyCatcher
(Plasmid #72327)

• Purpose: Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
• Depositing Lab: Mark Howarth
• Publication: Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5442
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pET21 MBPx-SpyCatcher
• Alt name: MBPx-SpyCatcher
• Species: Synthetic
• Insert Size (bp): 2661
• Mutation: MBP contains A312V, I317V mutations and 172, 173, 175 and 176 deletions
• Tag / Fusion Protein:
  • N-terminal His6 tag

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS analysis identifies a deletion of one nucleotide in the lacI sequence, relative to the expected backbone sequence. This would result in a truncation of the expected protein. The depositing laboratory has confirmed that this is of no substantial consequence.