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PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72326 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST14-SpyCatcher
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Backbone manufacturerHowarth lab (Addgene plasmid #35044)
- Backbone size w/o insert (bp) 6422
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAviTag-SpyCatcher
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SpeciesSynthetic
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Insert Size (bp)474
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Tag
/ Fusion Protein
- N-term His6 tag and TEV cleavage site
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AviTag-SpyCatcher was a gift from Mark Howarth (Addgene plasmid # 72326 ; http://n2t.net/addgene:72326 ; RRID:Addgene_72326) -
For your References section:
Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. 10.1073/pnas.1519214113 PubMed 26787909
