pET28a SpyCatcher-SnoopCatcher
(Plasmid #72324)

• Purpose: Bacterial expression of SpyCatcher linked to SnoopCatcher.
• Depositing Lab: Mark Howarth
• Publication: Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.

Backbone

• Vector backbone: pET21b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5442
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pET28a SpyCatcher-SnoopCatcher
• Alt name: SpyCatcher-SnoopCatcher
• Species: Synthetic
• Insert Size (bp): 774
• Mutation: 35 amino acids at the N-term of SpyCatcher have been deleted
• Tag / Fusion Protein:
  • N-terminal His6 tag

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the bacterial resistance of this plasmid is ampicillin, not kanamycin.