pET28a SnoopTag-MBP
(Plasmid #72323)

• Purpose: Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
• Depositing Lab: Mark Howarth
• Publication: Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pET28a SnoopTag-MBP
• Alt name: SnoopTag-MBP
• Species: Synthetic
• Insert Size (bp): 1233
• Tag / Fusion Protein:
  • N-terminal His6 tag. MBP is fused at the C-terminus of SnoopTag

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry