pET28a-SnoopCatcher
(Plasmid #72322)

• Purpose: Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.
• Depositing Lab: Mark Howarth
• Publication: Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pET28a-SnoopCatcher
• Alt name: SnoopCatcher
• Species: Synthetic
• Insert Size (bp): 405
• Mutation: changed G842T and D848G of S. pneumoniae adhesin RrgA (numbered according to PDB ID code 2WW8)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 tag (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry