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Addgene

pRK5-FLAG-FLCN
(Plasmid #72290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72290 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLCN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1740
  • GenBank ID
    NM_144997.5
  • Entrez Gene
    FLCN (a.k.a. BHD, DENND8B, FLCL)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer ATT TAG GTG ACA CTA TAG
  • 3′ sequencing primer TGT AAC CAT TAT AAG CTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-FLAG-FLCN was a gift from David Sabatini (Addgene plasmid # 72290 ; http://n2t.net/addgene:72290 ; RRID:Addgene_72290)

  • For your References section:

    The folliculin tumor suppressor is a GAP for the RagC/D GTPases that signal amino acid levels to mTORC1. Tsun ZY, Bar-Peled L, Chantranupong L, Zoncu R, Wang T, Kim C, Spooner E, Sabatini DM. Mol Cell. 2013 Nov 21;52(4):495-505. doi: 10.1016/j.molcel.2013.09.016. Epub 2013 Oct 3. 10.1016/j.molcel.2013.09.016 PubMed 24095279
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