Skip to main content
Addgene

FLCN-eGFP
(Plasmid #72289)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Clontech N1
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FLCN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1740
  • GenBank ID
    NM_144997.5
  • Entrez Gene
    FLCN (a.k.a. BHD, DENND8B, FLCL)
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CTA GCG CTA CCG GAC TCA
  • 3′ sequencing primer CACCATGGTGGCGACCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLCN-eGFP was a gift from David Sabatini (Addgene plasmid # 72289 ; http://n2t.net/addgene:72289 ; RRID:Addgene_72289)

  • For your References section:

    The folliculin tumor suppressor is a GAP for the RagC/D GTPases that signal amino acid levels to mTORC1. Tsun ZY, Bar-Peled L, Chantranupong L, Zoncu R, Wang T, Kim C, Spooner E, Sabatini DM. Mol Cell. 2013 Nov 21;52(4):495-505. doi: 10.1016/j.molcel.2013.09.016. Epub 2013 Oct 3. 10.1016/j.molcel.2013.09.016 PubMed 24095279
Related items:
Commonly requested with: