pCI SMN2
(Plasmid #72287)

• Purpose: SMN2 exon 7 splicing cassette
• Depositing Lab: Elliot Androphy
• Publication: Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96(11):6307-11.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMN2
• Alt name: survival of motor neuron 2, centromeric
• Species: H. sapiens (human)
• Insert Size (bp): 6840
• Mutation: contains exons 6-7-8 including introns, multiple SNPs compared to NG_008728.1
• Entrez Gene: SMN2 (a.k.a. BCD541, C-BCD541, GEMIN1, SMNC, TDRD16B)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: chim-int-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence detects multiple SNPs compared to the NCBI reference #NG_008728.1. The depositor confirmed that these mutations should not affect plasmid function.