Skip to main content
Addgene

pCI SMN1
(Plasmid #72286)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72286 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMN1
  • Alt name
    survival of motor neuron 1, telomeric
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6840
  • Mutation
    exons 6-7-8 including introns
  • Entrez Gene
    SMN1 (a.k.a. BCD541, GEMIN1, SMA, SMA1, SMA2, SMA3, SMA4, SMA@, SMN, SMNT, T-BCD541, TDRD16A)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer chim-int-F
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SMN1 contains C21123G, T21668C, G21721T, A27578C, A27660G, G27734A, T21595del, and potentially other SNPs compared to the closest reference sequence NG_008691.1.

Depositor states that these discrepancies should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI SMN1 was a gift from Elliot Androphy (Addgene plasmid # 72286 ; http://n2t.net/addgene:72286 ; RRID:Addgene_72286)

  • For your References section:

    A single nucleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy. Lorson CL, Hahnen E, Androphy EJ, Wirth B. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6307-11. PubMed 10339583
Related items:
Commonly requested with: