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Addgene

pJK561
(Plasmid #72283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 3954
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    thioredoxin
  • Alt name
    trxA
  • Alt name
    AZ09_01835
  • Alt name
    trx
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes cysteine-35 to serine
  • GenBank ID
    635177282 KDE21554
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codons are numbered starting from zero, in keeping with usual practice for thioredoxin sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK561 was a gift from T. J. Kappock (Addgene plasmid # 72283 ; http://n2t.net/addgene:72283 ; RRID:Addgene_72283)
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