Skip to main content
Addgene

pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)
(Plasmid #72273)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRS406
  • Backbone size w/o insert (bp) 4384
  • Total vector size (bp) 5273
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    3xFLAG tag and aa 83-231 of Mnase
  • Alt name
    3FLAG-MNase
  • Insert Size (bp)
    525
  • Promoter REB1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS) was a gift from Steven Henikoff (Addgene plasmid # 72273 ; http://n2t.net/addgene:72273 ; RRID:Addgene_72273)

  • For your References section:

    ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo. Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S. Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733. 10.1038/ncomms9733 PubMed 26490019
Related items:
Commonly requested with: