pJK588
(Plasmid
#72270)
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PurposeSynthetic gene corresponding to Synechocystis PCC6803 orf sll1489
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 3865
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Vector typeGene cloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehypothetical protein SYNGTS_3005
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Alt namesll1489
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Alt namecpmA
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SpeciesSynthetic; Synechocystis sp. PCC6803
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GenBank IDBAK51753
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- TEV cleavage site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK588 was a gift from T. J. Kappock (Addgene plasmid # 72270 ; http://n2t.net/addgene:72270 ; RRID:Addgene_72270)