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Addgene

pCDH-EF1-copGFP-T2A-Puro
(Plasmid #72263)

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Item Catalog # Description Quantity Price (USD)
Plasmid 72263 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCDH-CMV-MCS
  • Backbone manufacturer
    Systembio
  • Backbone size w/o insert (bp) 7133
  • Modifications to backbone
    ClaI/SalI fragment was replaced with EF1 + new MCS
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    copGFP-T2A-Puro
  • Insert Size (bp)
    1434
  • Promoter elongation factor-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GCACTTGATGTAATTCTCC
  • 3′ sequencing primer CAACACCACGGAATTGTCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-copGFP-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 72263 ; http://n2t.net/addgene:72263 ; RRID:Addgene_72263)
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