pGSKU
(Plasmid #72243)

• Purpose: Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter
• Depositing Labs: Michael Resnick, Francesca Storici
• Publication: Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 20.

Backbone

• Vector backbone: pFA6aKlURA3
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: KlURA3
• Alt name: URA3 gene from Kluyveromyces lactis

Gene/Insert 2

• Gene/Insert name: kanMX4
• Alt name: provides resistance to G418

Gene/Insert 3

• Gene/Insert name: I-SceI
• Alt name: I-SceI endonuclease
• Species: S. cerevisiae (budding yeast)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was created by Francesca Storici.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920