pCORE-Hp53
(Plasmid #72242)

• Purpose: Contains the CORE cassette with hyg and human p53 gene markers
• Depositing Labs: Michael Resnick, Francesca Storici
• Publication: Storici et al Genet Eng (N Y). 2003;25:189-207.

Backbone

• Vector backbone: pFA6aKlURA3
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: p53
• Alt name: cDNA of human p53 gene with the V122A mutation
• Species: H. sapiens (human)
• Mutation: V122A mutation
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Gene/Insert 2

• Gene/Insert name: hyg
• Alt name: provides resistance to hygromycin

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was created by Francesca Storici.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920