pcDNA4TO-Kif18b-24xGCN4_v1
(Plasmid
#72228)
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PurposeA fusion of the Kif18b motor protein to the v1 SunTag peptide array
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA4TO
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4TO-Kif18b-24xGCN4_v1 was a gift from Ron Vale (Addgene plasmid # 72228 ; http://n2t.net/addgene:72228 ; RRID:Addgene_72228) -
For your References section:
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933