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Addgene

pJK304
(Plasmid #72225)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23a
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4711
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    alanine racemase
  • Alt name
    alr
  • Alt name
    AZ09_RS09230
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes alanine-368 to aspartate
  • GenBank ID
    WP_042787947.1 ABC71322.1
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK304 was a gift from T. J. Kappock (Addgene plasmid # 72225 ; http://n2t.net/addgene:72225 ; RRID:Addgene_72225)

  • For your References section:

    Alanine racemase from the acidophile Acetobacter aceti. Francois JA, Kappock TJ. Protein Expr Purif. 2007 Jan;51(1):39-48. Epub 2006 Jun 6. 10.1016/j.pep.2006.05.016 PubMed 16843006
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