pGEX-6p-hDcp2 E147/8Q
(Plasmid #72217)

• Purpose: bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)
• Depositing Lab: Megerditch Kiledjian
• Publication: Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 6.

Backbone

• Vector backbone: pGEX-6p
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dcp2
• Alt name: decapping mRNA 2
• Species: H. sapiens (human)
• Mutation: E147Q, E148Q and F404L (please see depositor's comment below)
• Entrez Gene: DCP2 (a.k.a. NUDT20)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositor noted that the Dcp2 F404L mutation found in Addgene's quality control sequence does NOT affect plasmid function.