pET28a-hDcp2 E147Q, E148Q
(Plasmid
#72215)
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Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerEMD Biosciences
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameDcp2
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Alt namedecapping mRNA 2
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SpeciesH. sapiens (human)
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MutationE147Q, E148Q
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Entrez GeneDCP2 (a.k.a. NUDT20)
- Promoter T7
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-hDcp2 E147Q, E148Q was a gift from Megerditch Kiledjian (Addgene plasmid # 72215 ; http://n2t.net/addgene:72215 ; RRID:Addgene_72215) -
For your References section:
The hDcp2 protein is a mammalian mRNA decapping enzyme. Wang Z, Jiao X, Carr-Schmid A, Kiledjian M. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 6. 10.1073/pnas.192445599 PubMed 12218187