pCDNA3-Flag-hDcp2
(Plasmid
#72213)
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Purposemammalian expression of Flag-tagged human Dcp2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72213 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDcp2
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Alt namedecapping mRNA 2
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SpeciesH. sapiens (human)
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MutationQ170R (please see depositor's comment below)
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Entrez GeneDCP2 (a.k.a. NUDT20)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor noted that the Dcp2 Q170R mutation found in Addgene's quality control sequence does NOT affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-Flag-hDcp2 was a gift from Megerditch Kiledjian (Addgene plasmid # 72213 ; http://n2t.net/addgene:72213 ; RRID:Addgene_72213) -
For your References section:
The hDcp2 protein is a mammalian mRNA decapping enzyme. Wang Z, Jiao X, Carr-Schmid A, Kiledjian M. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 6. 10.1073/pnas.192445599 PubMed 12218187
