AB-GFP
(Plasmid #72203)

• Purpose: screen AB42 variants for aggregation
• Depositing Lab: Michael Hecht
• Publication: Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

Backbone

• Vector backbone: pET28(A+) based GFP folding reporter
• Backbone manufacturer: Waldo, et al. PMID 10404163
• Backbone size w/o insert (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AB42
• Alt name: amyloid beta (A4) precursor protein
• Alt name: APP
• Species: H. sapiens (human)
• Insert Size (bp): 123
• GenBank ID: 351
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: T7
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: GFP-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GFP folding reporter from Geoffrey Waldo-Los Alamos National Laboratory
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry