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Addgene

pMAL-ccDockerin
(Plasmid #72200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAL-c5G
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 5648
  • Total vector size (bp) 5872
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clostridium Cellulolyticum Type I Dockerin
  • Alt name
    CcDockerin
  • Species
    Clostridium Cellulolyticum
  • Insert Size (bp)
    224
  • Tags / Fusion Proteins
    • Maltose Binding Protein (N terminal on backbone)
    • His-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer DocSeq_F
  • 3′ sequencing primer DocSeq_R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL-ccDockerin was a gift from Timothy Whitehead (Addgene plasmid # 72200 ; http://n2t.net/addgene:72200 ; RRID:Addgene_72200)

  • For your References section:

    Determination of binding affinity upon mutation for type I dockerin-cohesin complexes from Clostridium thermocellum and Clostridium cellulolyticum using deep sequencing. Kowalsky CA, Whitehead TA. Proteins. 2016 Dec;84(12):1914-1928. doi: 10.1002/prot.25175. Epub 2016 Oct 26. 10.1002/prot.25175 PubMed 27699856
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