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PurposeFor mitochondrial unfolded protein response research
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4801
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOTC
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Alt nameOrnithine transcarbamylase
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Alt nameOrnithine carbamoyltransferase
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1464
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Entrez GeneOtc
- Promoter CAG
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pCAGGS5 (5'-ggttcggcttctggcgtgtgacc-3')
- 3′ sequencing primer rb_glob_PA_term-R (5'-CCCATATGTCCTTCCGAGTG-3) (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ornithine transcarbamylase (OTC) was a gift from Nicholas Hoogenraad (Addgene plasmid # 71877 ; http://n2t.net/addgene:71877 ; RRID:Addgene_71877) -
For your References section:
A mitochondrial specific stress response in mammalian cells. Zhao Q, Wang J, Levichkin IV, Stasinopoulos S, Ryan MT, Hoogenraad NJ. EMBO J. 2002 Sep 2;21(17):4411-9. PubMed 12198143
