pET-Sac-Abeta(M1-42)
(Plasmid #71875)

• Purpose: Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells
• Depositing Lab: Dominic Walsh
• Publication: Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10.1111/j.1742-4658.2008.06862.x.

Backbone

• Vector backbone: pET vector
• Backbone manufacturer: unknown
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Vector is a modified pET vector. Restriction digest sites for Abeta gene are Nde1 (5') and Sac1 (3')
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Abeta(M1-42)
• Alt name: amyloid-beta 42
• Alt name: abeta 42
• Species: H. sapiens (human)
• Insert Size (bp): 129
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: Sac1 (not destroyed)
• 5′ sequencing primer: 5'- ATG GAC GCT GAA TTC CGT CAC GAC TCT GGT TAC GAA GTT CAC CAC CAG AAG CTG GTG -3'
• 3′ sequencing primer: 5'- GTG GGT TCT AAC AAG GGT GCT ATC ATC GGT CTG ATG GTT GGT GGC GTT GTG -3'

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry