pGFP-Kif5cmut
(Plasmid #71854)

• Purpose: mammalian expression of mutant GFP-Kif5c
• Depositing Lab: Michelle Peckham
• Publication: Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95. doi: 10.1242/jcs.026492. Epub 2008 Mar 11.

Backbone

• Vector backbone: pEGFPC3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif5c
• Alt name: kinesin family member 5C
• Species: R. norvegicus (rat)
• Insert Size (bp): 3000
• Mutation: T93N mutation and also a novel SpeI site (1652). Kif5c starts at AA3.
• Entrez Gene: Kif5c
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • GFP-kif5cVector

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry