pGFP-Kif5c
(Plasmid #71853)

• Purpose: mammalian expression of GFP-Kif5c
• Depositing Lab: Michelle Peckham
• Publication: Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95. doi: 10.1242/jcs.026492. Epub 2008 Mar 11.

Backbone

• Vector backbone: pEGFPC3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif5c
• Alt name: kinesin family member 5C
• Species: R. norvegicus (rat)
• Insert Size (bp): 3000
• Mutation: Kif5c starts at AA3
• Entrez Gene: Kif5c
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • GFP-kif5cVector
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Following transfection, look at cells that have low levels of expression (for the wild type Kif5c protein) to investigate dynamic behaviour, as high levels of expression result in bundling of microtubules.