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Addgene

pMES140
(Plasmid #71849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71849 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Total vector size (bp) 7000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NME1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    NME1 (a.k.a. YNCN0013W)
  • Promoter NME1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CEN, LEU2 based plasmid with NME1 gene region sufficient for NME1 expression in yeast where NME1 gene is disrupted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMES140 was a gift from David Clayton & Mark Schmitt (Addgene plasmid # 71849 ; http://n2t.net/addgene:71849 ; RRID:Addgene_71849)

  • For your References section:

    Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae. Schmitt ME, Clayton DA. Mol Cell Biol. 1993 Dec;13(12):7935-41. PubMed 8247008
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