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Addgene

pJK340
(Plasmid #71837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC118
  • Backbone size w/o insert (bp) 3162
  • Total vector size (bp) 3719
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide mutase
  • Alt name
    purE
  • Alt name
    AZ09_02690
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes histidine-59 to asparagine
  • GenBank ID
    AAZ04483.1 KDE21029.1
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13-forward
  • 3′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The 5‘-UTR contains a 43 bp region between the A. aceti (not E coli as in the publication) lpxH and purE genes, which is presumed to harbor the endogenous promoter region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK340 was a gift from T. J. Kappock (Addgene plasmid # 71837 ; http://n2t.net/addgene:71837 ; RRID:Addgene_71837)

  • For your References section:

    Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti. Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ. Biochemistry. 2006 Jul 11;45(27):8193-208. 10.1021/bi060465n PubMed 16819818
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