pJK340
(Plasmid #71837)

• Purpose: Cloned region contains Acetobacter aceti 1023 purE(H59N) gene
• Depositing Lab: T. J. Kappock
• Publication: Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-208.

Backbone

• Vector backbone: pUC118
• Backbone size w/o insert (bp): 3162
• Total vector size (bp): 3719
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide mutase
• Alt name: purE
• Alt name: AZ09_02690
• Species: Acetobacter aceti 1023
• Mutation: changes histidine-59 to asparagine
• GenBank ID: AAZ04483.1 KDE21029.1
• Promoter: lac

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13-forward
• 3′ sequencing primer: M13-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 5‘-UTR contains a 43 bp region between the A. aceti (not E coli as in the publication) lpxH and purE genes, which is presumed to harbor the endogenous promoter region.