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Addgene

pJK193
(Plasmid #71834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lambda-BlueStar
  • Backbone size w/o insert (bp) 2111
  • Total vector size (bp) 14764
  • Vector type
    phage lambda cloning vector with genomic DNA insert

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide mutase
  • Alt name
    purE
  • Alt name
    AZ09_02690
  • Species
    Acetobacter aceti 1023
  • Mutation
    additional N-terminal methionine encoded by this plasmid
  • GenBank ID
    AAZ04483.1 KDE21029.1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sau3AI (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer T3 promoter
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide synthetase
  • Alt name
    purK
  • Alt name
    AZ09_02695
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    AAZ04484.1 KDE21030.1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site Sau3AI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK193 was a gift from T. J. Kappock (Addgene plasmid # 71834 ; http://n2t.net/addgene:71834 ; RRID:Addgene_71834)

  • For your References section:

    Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti. Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ. Biochemistry. 2006 Jul 11;45(27):8193-208. 10.1021/bi060465n PubMed 16819818
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