pJK193
(Plasmid #71834)

• Purpose: Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
• Depositing Lab: T. J. Kappock
• Publication: Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-208.

Backbone

• Vector backbone: lambda-BlueStar
• Backbone size w/o insert (bp): 2111
• Total vector size (bp): 14764
• Vector type: phage lambda cloning vector with genomic DNA insert

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide mutase
• Alt name: purE
• Alt name: AZ09_02690
• Species: Acetobacter aceti 1023
• Mutation: additional N-terminal methionine encoded by this plasmid
• GenBank ID: AAZ04483.1 KDE21029.1

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sau3AI (not destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: T3 promoter
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: N5-carboxyaminoimidazole ribonucleotide synthetase
• Alt name: purK
• Alt name: AZ09_02695
• Species: Acetobacter aceti 1023
• GenBank ID: AAZ04484.1 KDE21030.1

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: Sau3AI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 promoter

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
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