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Addgene

pJK348
(Plasmid #71832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC118
  • Backbone size w/o insert (bp) 3162
  • Total vector size (bp) 5247
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hypothetical protein
  • Alt name
    orfX
  • Alt name
    AAZ04482
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    AAZ04482.1 KDE21028.1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide mutase
  • Alt name
    purE
  • Alt name
    AZ09_02695
  • Species
    Acetobacter aceti 1023
  • Mutation
    changes histidine-59 to asparagine
  • GenBank ID
    AAZ04483.1 KDE21030.1

Gene/Insert 3

  • Gene/Insert name
    N5-carboxyaminoimidazole ribonucleotide synthetase
  • Alt name
    purK
  • Alt name
    AZ09_02690
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    AAZ04484.1 KDE21030.1

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13-forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Oriented opposite to lac promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK348 was a gift from T. J. Kappock (Addgene plasmid # 71832 ; http://n2t.net/addgene:71832 ; RRID:Addgene_71832)

  • For your References section:

    Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti. Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ. Biochemistry. 2006 Jul 11;45(27):8193-208. 10.1021/bi060465n PubMed 16819818
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