pET28-Dnmt3a3L-sc27
(Plasmid #71827)

• Purpose: For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
• Depositing Lab: Albert Jeltsch
• Publication: Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.

Backbone

• Vector backbone: pET28a
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion
• Alt name: Mouse Dnmt3a catalytic domain
• Alt name: Mouse Dnmt3L C-terminal domain
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Entrez Gene: Dnmt3a (a.k.a. MmuIIIA)
• Tag / Fusion Protein:
  • 6xHis tag (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Gene origin for Dnmt3a full length was Dr. En Li (Cardiovascular Research Center, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Charlestown, MA 02129, USA).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For protein production, use BL21 Rosetta (DE3) cells.